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'noninvasive diagnostic method'

Items tagged with 'noninvasive diagnostic method'

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Rapid Test

Buy AccuTest H Pylori AB Rapid Card Test (50 Tests) Online from Accurex. The Helicobacter Pylori is a lateral flow chromatographic immunoassay for the qualitative detection of antibodies to H.pylori in human Whole Blood/Serum/Plasma. H Pylori test provides an aid in the diagnosis of infection with H.pylori. H.pylori is implicated in the etiology of a variety of gastrointestinal diseases, including duodenal and gastric ulcer, non-ulcer dyspepsia and active and chronic gastritis. h pylori symptoms are an ache or burning pain in your stomach (abdomen), Nausea, Loss of appetite, Frequent burping, Bloating & Unintentional weight loss NTENDED USE The H.pylori Antibody Rapid Test Card is a lateral flow chromatographic immunoassay for the qualitative detection of antibodies to H.pylori in human Whole Blood/Serum/Plasma. It provides an aid in the diagnosis of infection with H.pylori. INTRODUCTION H.pylori is implicated in the etiology of a variety of gastrointestinal diseases, including duodenal and gastric ulcer, non-ulcer dyspepsia and active and chronic gastritis. H.pylori infection is found in more than 90% of duodenal ulcer patients and in around 75% of all peptic ulcer sufferers. H.pylori infection is also more common in gastric cancer patients. The risk of gastric cancer has been estimated to be six-fold higher in H.pylori infected populations than in uninfected populations. H.pylori infections occur in human populations throughout the world. In developed countries, about 50% of the population may have H.pylori infection by the age of 60 years, while only 10-20% of adults in the third decade of life have it. Transmission is most probably by the fecal-oral or oral-oral route. Both invasive and non-invasive methods are used to diagnose H.pylori infection in patients with symptoms of gastrointestinal disease. Invasive methods include culture of gastric biopsy samples, histologic examination of stained biopsy specimens, or direct detection of the urease activity in the biopsy (CLO test). These methods need to obtain a biopsy sample by endoscopy.which is expensive,and usually results discomfort and risk to the patient.noninasive techniques include urea breath tests and serological methods. Urea breath test requires expensive laboratory equipment and moderate radiation exposure. Serologic tests are employed to detect antibodies as human immune response to H.pylori, for example the ELISA and the Western immunoblot. The H.pylori Antibody Rapid Test Card detect antibodies to H.pylori infection in human Whole Blood/Serum/Plasma. It is a noninvasive method and does not use radioactive isotopes. The test is easy to perform and requires no specialized equipment. Visual interpretation provides an accurate qualitative result. It is a useful on-site aid in the diagnosis of H.pylori infection. Diagnosis of H.pylori infection by antibody immunoassay can reduce the number of patients requiring endoscopy. PRINCIPLE The H.pylori Antibody Rapid Test Card is an immunoassay based on the principle of the double antigen-sandwich technique. During testing, a Whole Blood/Serum/Plasma specimen migrates upward by capillary action. The antibodies to H.pylori if present in the specimen will bind to the H.pylori conjugates. The immune complex is then captured on the membrane by the pre-coated H.pylori antigens, and a visible pink-purple line will show up in the test line region indicating a positive result. If antibodies to H.pylori are not present or are present below the detectable level, a pink-purple line will not form in the test line region indicating a negative result. To serve as a procedural control, a pink-purple line will always appear at the control line region, indicating that proper volume of specimen has been added and membrane wicking has occurred. Safety Information The H.pylori Antibody Rapid Test Card is limited to provide a qualitative detection. The intensity of the test line does not necessarily correlate to the concentration of the antibody in the blood. x The results obtained from this test are intended to be an aid in diagnosis only. Each physician must interpret the results in conjunction with the patient’s history, physical findings, and other diagnostic procedures. x A negative test result indicates that antibodies to H.pylori are either not present or at levels undetectable by the test. Direction for Use TEST PROCEDURE 1. Remove the test cassette from the sealed pouch and use it as soon as possible. 2. Place the test cassette on a clean and level surface. 3. For serum or plasma specimen: Hold the dropper vertically and transfer 3 drops of serum or plasma to the specimen well (S) of the test cassette, then start the timer. See illustration below. 4. For whole blood specimens: Hold the dropper vertically and transfer 1 drop of whole blood (approximately 30μl) to the specimen well(S) of the test cassette, then add 2 drops of buffer (approximately 70μl) and start the timer. 5. Read results at 15 minutes. Do not interpret the result after 20 minutes. Other Info STORAGE AND STABILITY x Store as packaged in the sealed pouch at 4-40℃. The kit is stable within the expiration date printed on the label. x Once open the pouch, the test should be used within one hour. Prolonged exposure to hot and humid environment will cause product deterioration.

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Clinical Chemistry

Infinite Liquid a-Amylase 12 ml Diagnostic Reagent from Accurex (Pack Size : 6 x 2 ml). a-Amylase reagent is used for determination of amylase activity based on kinetic method using Gal G2 – a CNP. Amylase is a ready-to-use reagent. Normal amylase level is 40 to 140 units per liter (U/L) or 0.38 to 1.42 microkat/L (µkat/L). Infinite a-Amylase can be used on any Spectrophotometer, Discrete semiautomated & Automated analyzers. Reaction type is Kinetic. Accurex Biomedical is the first biochemical reagents manufacturing company in India About Infinite a-Amylase ‘is a reagent set for determination of amylase activity based on kinetic method using Gal G2 – a CNP. Infinite a-Amylase is a ready-to-use reagent. Infinite a-Amylase can be determined in just 21/2 minutes at 37°C. Infinite a-Amylase is linear upto 2000 IU/1. Infinite a-Amylase is a High Stability Reagent. Infinite a-Amylase can be used on any Spectrophotometer, Discrete semiautomated and Automated analyzers. Programme can be designed for any specific analyzer upon request. Pack Size : 6 x 2 ml Principle Amylase test involves use of a chromogenic substrate Gal G, – a CNP (2-chloro-4-nitrophenyl linked with galactosyl maltoside). The direct action of amylase with this substrate results in the release of more than 90% of 2-chloro-4-nitrophenol, which can be monitored by kinetic assay at 405 nm. The increase in absorbance is directly proportional to the amylase activity in the sample. Quality Control To ensure adequate quality control, it is recommended that each batch should include a normal and abnormal commercial reference control serum. It should be realized that the use of quality control material checks both instrument and reagent functions together. Factors which might affect the performance of this test include proper instrument function, temperature control, cleanliness of glassware and accuracy of pipetting. Other Details Mix thoroughly and transfer the assay mixture immediately to the thermostated cuvette and start the stopwatch simultaneously. Record the first reading at 60 second and subsequently three more readings with 30 seconds interval at 405 nm.

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Clinical Chemistry

Autozyme Alkaline Phosphatase 60 Diagnostic Reagent (Pack Size : 12 x 5 ml) from Accurex. Alkaline Phosphatase 60 are used in the diagnosis of hepatobiliary disorders and bone disease associated with increased osteoblastic activity. Alkaline Phosphatase high levels may be a sign of a liver problem or a bone disorder Alkaline Phosphatase 60 reagent is set for determination of Alkaline Phosphatase activity based on kinetic method using p-nitrophenyl phosphate (p-NPP), the reagent is a Highly stable reagent & its activity can be determined in just 2.5 minutes. About Autozyme Alkaline Phosphatase is a reagent set for determination of Alkaline Phosphatase activity based on kinetic method using p-nitrophenyl phosphate (p-NPP) Autozyme Alkaline Phosphatase is a single reagent system using one step procedure Autozyme Alkaline Phosphatase has one step reconstitution. It involves dissolving substrate tablet in diluent Autozyme Alkaline Phosphatase is a Highly stable reagent Autozyme Alkaline Phosphatase activity can be determined in just 2.5 minutes Pack Size : 12 x 5 ml Principle The working solution is stablə for 21 days at 2- 8°C. The working solution should be prepared and stored in the dark (working solution bottle) provided.This is critical because the reagent is light sensitive. It should therefore be kept away from direct light. Alkaline Phosphatase cleaves p-nitrophenyl phosphate (p-NPP) into p-nitrophenol and phosphate. p-nitrophenol is a yellow color compound in alkaline medium and absorbs light at 405 nm. The rate of increase in absorbance at 405 nm. is proportional to Alkaline phosphatase activity in specimen. Quality Control To ensure adequate quality control, it is recommended that each batch should include normal and abnormal commercial reference control serum. It should be realized that the use of quality control material checks both instrument and reagent functions together. Factors which might affect the performance of this test include proper instrument function, temperature control, cleanliness of glassware and accuracy of pipetting. Other Details The kit should be stored at 2-8°C and is stable till the expiry date indicated on the label. The working solutions of Non Prostatic Acid Phosphatase & Total Acid Phosphatases are stable for 7 days at 2-8°C.

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Clinical Chemistry

Autozyme Alkaline Phosphatase 300 Diagnostic Reagent from Accurex (Pack Size : 30 x 10 ml). Alkaline Phosphatase reagent is used for determination of Alkaline Phosphate activity based on kinetic method using p-nitrophenyl phosphate (p-NPP). Alkaline Phosphatase is a single reagent system using one step procedure & Alkaline Phosphatase activity can be determined in just 2.5 minutes & is is a Highly stable reagent. Alkaline Phosphatase cleaves p-nitrophenyl phosphate p- NPP in the presence of magnesium ions to form p nitrophenol & phosphate. About Method – p-NPP-DEA Buffer – Kinetic reaction with 60 seconds lag phase – Easy reconstitution : Tablet + Diluent – High liquid stability : 21 days Autozyme Alkaline Phosphatase is a reagent set for determination of Alkaline Phosphate activity based on kinetic method using p-nitrophenyl phosphate (p-NPP) Autozyme Alkaline Phosphatase is a single reagent system using one step procedure Autozyme Alkaline Phosphatase has one step reconstitution. It involves dissolving substrate tablet in diluent Autozyme Alkaline Phosphatase is a Highly stable reagent Autozyme Alkaline Phosphatase activity can be determined in just 2.5 minutes Principle Alkaline Phosphatase cleaves p-nitrophenyl phosphate p- NPP) in the presence of magnesium ions to form p nitrophenol and phosphate. The intensity of the yellow color formed is directly proportional to the Application alkaline phosphatase activity in the specimen and measured kinetically. Quality Control To ensure adequate quality control, it is recommended that each batch should include normal and an abnormal commercial reference control serum. It should be realized that the use of quality control material checks both instrument and reagent functions together. Factors which might affect the performance of this test include proper instrument function, temperature control, cleanliness of glassware and accuracy of pipetting. Other Details Two liquid reagent systems. Stable till the expiry date indicated on the label when stored in 2-8°C.

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Clinical Chemistry

utozyme Albumin 300 Diagnostic Reagent from Accurex. Albumin 300 is used for determination of albumin in serum or plasma based on BCG method. Autozyme Albumin is a single reagent system which is ready to use, Albumin is linear up to 6 gm% & can be determined in 1 minute at room temperature. Serum albumin in the presence of Bromocresol-green under acidic condition forms a green coloured complex. Albumin normal range is 3.4 to 5.4 g/dL (34 to 54 g/L). About Autozyme Albumin is a reagent set for determination of albumin in serum or plasma based on BCG method Autozyme Albumin is a single reagent system, ready to use. Autozyme Albumin is linear up to 6 gm% Albumin can be determined in 1 minute at room temperature The shelf-life of Autozyme Albumin is 18 months Pack Size : 2 x 50 ml Principle Serum albumin in the presence of Bromocresol-green under acidic condition forms a green coloured complex, The absorbance of this complex is proportional to the albumin concentration in serum or plasma. Quality Control To ensure adequate quality control, it is recommended that each batch should include a normal and an abnormal commercial reference control serum,. it should be realized that the use of quality control material checks both instrument and reagent functions together. Factors which might affect the performance of this test include proper instrument function, cleanliness of glassware and accuracy of pipetting. Other Details The reagents are for in vitro diagnostic use. BCG reagent and standard should be stored at temperature indicated on the bottle label.

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Clinical Chemistry

Acid Phosphatase Diagnostic Reagent (Pack size: 8 x 1 ml) from Accurex. Acid Phosphatase is used in accordance with ISO 14189 to detect acid phosphatase from Clostridium perfringens. Autozyme Acid Phosphate reagent is set for determination of Acid Phosphatase activity based on Kinetic method using a-naphthyl phosphate. In acidic pH of the buffer system, the reagent hydrolyses a-naphthyl phosphate to a-naphthol & phosphate. Accurex has gained considerable expertise in manufacturing & marketing of In-vitro Diagnostic reagents. About Autozyme Acid Phosphate is a reagent set for determination of Acid Phosphatase activity based on Kinetic method using a-naphthyl phosphate Autozyme Acid Phosphate is a single reagent system using one step procedure Autozyme Acid Phosphate can be determined in just 8 minutes at 37 degree C Acid Phosphate can be used on any Spectrophotometer, Discrete semi automated and Automated Analyzer. Programme can be designed for any specific analyzer upon request. Pack Size : 8 x 1 ml Principle In acidic pH of the buffer system, Acid Phosphatase hydrolyses a-naphthyl phosphate to a-naphthol and phosphate. The o-naphthol is then coupled with Diazotized Fast Red TR to form a Diazo dye which has strong absorbance at 405 nm. The increase in absorbance is directly proportional to the level of acid phosphatase in serum. The addition of L-Tartrate inhibits prostatic acid phosphatase but does not inhibit other isoenzymes. The activity of Prostatic Acid Phosphatase is obtained by subtracting the result of Non prostatic Acid phosphatase (Determination with Tartrate) from Total Acid Phosphatase (without tartrate). Quality Control To ensure adequate quality control it is recommended that each batch should include a normal and an abnormal commercial reference control serum. It should be realized that the use of quality control material checks both instrument and reagent functions together. Factors which might affect ne performance of this test includes proper instrument function, temperature control, cleanliness of glassware and accuracy of pipetting.

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Rapid Test

Intended Use Diagnos Dengue card is a rapid solid phase Immuno-chromatographic test for the qualitative differential detection of IgM and IgG antibodies to dengue virus in Human serum /Plasma. This test is for in vitro diagnostic use only and is intended as an aid in for presumptive diagnosis between primary and secondary dengue infection. Salient Features Rapid, visual and qualitative Immunoassay. Differential detection of IgM & IgG Antibodies. Detection of all the 4 Dengue serotypes (DEN-1, DEN-2, DEN-3 and DEN-4). See through device for easy interpretation of results. Unique sample processing method ensures highest accuracy in results. Results with in 20 minutes. Longer shelf life : 30 months at 2-30°C. Convenient pack size : 10 tests Sensitivity-95% and Specificity- 97%. Principle

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Rapid Test

STANDARD Q Ultra Dot HCV Standard Q HCV Ab Test is a rapid chromatographic immunoassay for the qualitative detection of specific antibodies to HCV present in human serum,plasma or whole blood.This test is for in vitro professional diagnostic use and intended as an aid to early diagnosis of HCV infection in patient with clinical symptoms with HCV infection.It provides an initial screening test result.More specific alternative diagnosis methods should be performed in order to obtain the confirmation of HCV infection.

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