EM 360 is a Fully Automated Clinical Chemistry Analyser which is time tested and proven technology. It is designed to provide the most demanding productivity, speed and operational ease. EM 360 with a throughput of 360 tests/hour offers flexibility in operation and rugged performance to win against high workload, tight deadlines, multiple tasks and complexity of testing. It yields high quality results for virtually any biochemistry test to deliver quick, efficient and consistent performance. Product Features: Automated, Discrete, Patient prioritized, Floor model Based on proven technology, Diffraction Grating Separate probes for Reagent R1 & R2 and dedicated probe for sample Low reading volume of 200 µl only Onboard cooling for reagents to enhance the reagent stability Barcode for reagents & samples (Optional) Wide test menu Product Applications: End Point Reactions: Glucose, Cholesterol, Triglycerides, Albumin, Total Protein, Uric Acid, Calcium, Magnesium, Bilirubin Total, Bilirubin Direct Kinetic Reactions: SGOT, SGPT, GGT, LDH, CK, CK MB, ALP, ACP, Amylase, Lipase Two Point Rate Reactions: Urea, Creatinine Immunoturbidimetric Chemistries: RA, CRP, ASO, Direct HDL, Direct LDL, Apo A, ApoB, Lp (a) Special Chemistries: HbA1c, Iron, Ferritin, Phosphorus, Microprotein, Ammonia, Bicarbonates System Type: Discrete, automated, random access, patient prioritized clinical chemistry analyzer Throughput: 360 tests / hour photometric and 600 tests / hour with ISE* (*optional ISE with Na+, K+, Cl-, Li+) Analytical Methods: 1-Point, 2-Point, Rate-A, Rate-B, Direct Potentiometry (optional) Barcode Reader: For reagents and samples (optional) Reaction Tray: 60 hard glass cuvettes Reaction Mixing: Stirrer with variable speed Minimum reaction Volume: 150 μl with maximum 200 μl reading volume On-board Laundry: 5 stage cleaning, 2 stage drying Photometer: Multi-wavelength diffraction grating with 12 wavelengths (340, 376, 415, 450, 480, 505, 546, 570, 600, 660, 700, 750 nm) OD Range: 0.0 - 3.0 Light Source: Halogen lamp Detector: Silicon photo-diode Water Consumption: < 10 litres Programmable Parameters: Default system pack parameters + upto 99 user defined parameters, Unlimited profile and unlimited calculation items Quality Control: QC plot data with QC rules. Provision for lab mean. Twin plot Calibration: K-Factor, Linear (1, 2 point & multipoint), 4P and 5P Logit-log, cubic spline, exponential, polynomial, On-board serial dilution for calibrator Power Requirement: AC 220 V ± 10 %, 50 Hz or AC 110 V ± 10 %, 60 Hz Power Consumption: 800 VA Dimension (mm): Approx. 675 (W) x 840 (D) x 1120 (H) Weight: Approx. 150 kgs. SAMPLE HANDLING Sample: Serum, Plasma, Urine, CSF, Whole blood, other Sample Unit: 82 positions for samples, blank , controls, calibrators, STAT sample Sample Pipetting: 2 - 70 µl (adjustable in 0.1 µl step) for Biochemistry, 70 µl fixed for ISE Capacitance probe with liquid level sensing & vertical obstruction detection, serum indices Auto Rerun: Repeat with same, increased or decreased volume ( upto 1:150) Sample Tubes /Cups: Primary tubes of 5 ml, 7 ml, 10 ml and sample cups REAGENT HANDLING Reagent Tray: 50 positions for reagents with onboard cooling Reagent Pipetting: R1: 50 - 300 µl (adjustable in 1 µl step) R2: 10 - 200 µl (adjustable in 1 µl step) Capacitance probe with level sensing & vertical obstruction detection SYSTEM INTERFACE: Analyzer-PC: USB PC - Host Computer: Bidirectional TCP / IP & RS - 232 PC - Printer: USB Operating System: Windows Based Database: Unlimited Results

Fully Automated Chemiluminescence Immunoassay Analyzer Introducing iFlash 1200, a chemiluminescence immunoassay analyzer that offers fully automated immunoassay analysis using Magnetic particle-based Direct Chemiluminescence technology. With a high throughput of up to 120 tests per hour and a first result available in just 13 minutes, iFlash 1200 is one of the fastest and most efficient immunoassay analyzers available on the market. The instrument features continuous STAT sample loading capacity with 16 sample positions, continuous reagents loading capacity with 15 reagent positions, and continuous loading of reaction vessels with a capacity of 500 cuvettes. Additionally, iFlash 1200 uses Acridinium Ester Labeled Direct Chemiluminescence for highly accurate and reliable results, and comes with ready-to-use reagents in convenient 2 x 50 tests pack size for simplified testing. Overall, iFlash 1200 is an innovative and versatile immunoassay analyzer that is perfect for laboratories that require high throughput and rapid turnaround times. iFlash 1200 chemiluminescence immunoassay analyzer Key Features: 1.Continuous loading of reagents with convenient pack size of 2 x 50 tests 2.Continous loading of 500 reaction cuvettes for longer walkaway time 3. 4 step magnetic separation for achieving high precision and quality results 4.Non contact vortex mixer-Highly Efficient technology to prevent carryover & cross contamination Additional information General Information Throughput : upto 120 tests/hour Time to First Results :13 minutes Detection Principle Magnetic particle Based Direct Chemiluminescence Chemiluminescence Label :Acridinium Ester Sample System Sample Position :16 sample position Types of Sample tube :Microcup & blood collecting tubes Sample Volume :5~150μL Reagent System Reagent Position :15 reagent positions with onboard refrigeration Reagent Carousel Temp :2-8 °C Reagent Detection :Reagent Level Tracking Reagent Pack : 2 x 50 tests Reagent Carousel Incubation Capacity :48 reaction vessels Incubation Temperature :37°C+-0.3°C Mixing System :Non-Contact Vortex Mixer Operating Conditions Power Supply :100~240V, 50/60HZ Power Consumption :600VA Temperature :10~30°C Weight :120 Kg Reagents iFlash 1200 reagents iFlash 1200 reagents Pack size 2 x 50 Tests 28 days On board reagent stability 28 days Calibration stability Built in Calibrators for all Parameters

Product Features: Better precision, CV < 2.5 % Patented CLE technology enables complete elimination of labile glycohemoglobin ensuring accurate HbA1c results. HbA1c test results in 3.5 minutes with HbA2/HbF flagging. Separation of hemoglobin fractions Complete removal of labile fraction up to 10% No interference of HbF up to 10% No interference of lipemia or icterus, can run on non-fasting patient samples HbA1c reportability presence of HbS, HbC, HbD and HbE Compact bench top model User friendly touch screen operation Sampling from multiple sized primary tubes, including microcapillary collection tubes On board QC functions with Levey-Jennings and statistical calculations Unlimited storage of calibration, QC data and chromatograms No sample preparation required Simple reagent and column replacement Automated maintenance RFID reagent identification and tracking Automatic barcode identification of samples NGSP certified Calibrators and Controls IFCC traceable Complete kit with buffers, calibrator and column Product Application: Hba1c Testing Sampler Handler: 10 Position Rack Loader, positive identification by internal barcode reader Sample Type: Whole blood on EDTA K3 or K2 anticoagulant Haemolysate from EDTA whole blood samples Time for Analysis: Throughput approximately 3.5 minutes between samples Interferences: Complete removal (not separation) of labile up to 10% Sampling System: Sample Aspiration Probe coupled with a high precision, Sample Aspiration Volume: 14.5 µl in whole blood, 670 µl in lysate mode. Pump: 2 high pressure syringe pumps Detector Type: UV-Vis Reagent Traceability: RFID Printer: Built- in thermal printer with possibility to connect to external printer

Product Description: XL-640 with CRS is a random access, discrete, patient prioritized, fully automated clinical chemistry analyzer with autoloader designed to meet the needs of today’s modern clinical laboratories with high workload. Once samples are loaded, it is a complete walk-away system. XL 640 CRS is designed to operate smoothly over the long haul and to deliver consistent quality report. Product Features: Throughput of 400 tests/ hour (Photometric) and 640 tests/hour with ISE 56 Reagent Positions with On-board cooling for reagents 162 Sample positions with /Autoloader Separate probes for reagent R1 & R2 and dedicated probe for sample Clot detection for sample probe, Reflex testing and Serum Indices identification 72 permanent hard glass cuvettes with On-board Laundry Automatic dilution for high abnormal samples & Auto Rerun facility Extensive QC Programmes with Levy –Jennings Curve & QC rules. Automatic calculation for Mean SD & CV% Direct loading for primary tubes Touch screen User Friendly GUI with Bi-directional connectivity. System General System Type: Automated, Discrete, Random Access, Patient Prioritized Clinical Chemistry Analyzer with Autoloader Throughput: 400 photometric tests/hour & 640 tests/hour with ISE (*optional ISE with Na, K, Cl, Li) Analytical Methods: 1-Point, 2-Point, Rate-A, Rate-B, Direct Potentiometry(optional) Barcode Reader: Both for Samples & Reagents (Optional) Reactions Tray: 72 hard glass cuvettes Reaction Mixing: Dual Stirrer with variable speed Reading Volume: 180 µl On-Board Laundry: 6 stage cleaning, 2 stage drying Photometer: Multi-wavelength diffraction grating with 12 wavelengths (340, 376, 415, 450, 480, 505, 546, 570, 600, 660, 700 & 750 nm) O.D. Range: 0 - 3.0 Light Source: Halogen Lamp Detector: Silicon Photodiodes Water Consumption: < 14 liters/hour Programmable Parameters: Default system pack parameters + upto 99 user defined parameters, Unlimited profiles and unlimited calculation items Quality Control: Q.C. plot data with Q.C. Rules. Provision for lab mean. Twin plot. Caliberation: K- factor, Linear (1,2 point and multipoint) 4P and 5P Logit-log, Cubic spline,exponential, polynomial. On-board serial dilution for calibrator Power Requirement: AC 220 V ± 10 %, 50 Hz or AC 110 V ± 10 %, 60 Hz Power Consumption: 800 VA Dimensions: Approximately 897 mm (w) x 655 mm (D) x 1170 mm (H) Weight: Approximately 200 Kg Sample Handling Sample: Serum, Plasma, Urine, CSF, Whole blood, other Sample Unit: upto 162 positions for samples/STAT/calibrator/controls with Autoloader Sample Pipetting: 2-70 µl (adjustable in 0.1 µl steps) for biochemistry,70 µl fixed for ISE capacitance probe with liquid level sensing and vertical obstruction detection , clot detection and serum indices and Reflex Testing Auto Rerun: Repeat with same, increade and decresaed volume (upto 1:150) Sample Tubes/Cups: Primary tubes of 5 ml, 7ml and 10 ml and sample cups Reagent Handling Reagent Tray: 56 positions for reagents with on-board cooling Reagent Pipetting: R1: 60 - 300 µl (Adjustable in 1 µl step) R2: 0 or 10 - 300 l (Adjustable in 1 µl step) Capacitance probe with level sensing and vertical obstruction detection System Interface Analyzer-PC: USB PC- HOST Computer: Bidirectional TCP/IP and RS - 232 PC- Printer: USB Operating System: Windows based with Touch Screen User Friendly GUI Database: Unlimited Results

ADVIA 2400 Chemistry System The right balance of speed and efficiency The ADVIA® 2400 Clinical Chemistry System manages the most demanding workloads and meets high turnaround-time (TAT) goals. Its menu includes methods for testing drugs-of-abuse, therapeutic drugs (TDM) and new assays such as cystatin C and CardioPhase® hsCRP. The ADVIA 2400 Clinical Chemistry System offers: High throughput of up to 2400 tests/hour (1800 photometric and 600 ISE) to manage heavy workloads Rapid, consistent, 2-second cycle time for faster tube speed and test throughput User-defined automatic repeat, dilution, and reflex testing Large onboard reagent capacity and optional concentrated reagents help to minimize intervention demands Refrigerated onboard storage of controls and reagents for extended stability and increased productivity Automation-ready design: no additional hardware required Features & Benefits The ADVIA 2400 Clinical Chemistry System meets today’s high turnaround-time goals with a throughput of up to 2400 tests/hour. In addition to assays for general and specialty chemistries, its menu includes assays for specific protein measurement, drugs-of-abuse testing (DAT), and therapeutic drug monitoring (TDM). Interesting new assays such as cystatin C, HbA1c with automated sample pretreatment, and CardioPhase® hsCRP can now be included in your routine testing. The ADVIA 2400 Chemistry System pushes productivity levels while maintaining the reliability and stability that keep laboratories up and running. A rapid, 2-second probe cycle time maximizes tube speed and test throughput and combines with the system’s efficient automation-readiness to provide the turnaround time needed for high-volume laboratories Superior Speed and Productivity Throughput of up to 2400 tests/hour (1800 photometric and 600 ISE) to manage the heaviest workloads Rapid, consistent, 2-second cycle time Throughput of 200 tubes per hour, even when running three ISE tests and nine photometric tests per sample Automatic Sample Retain technology, auto-repeat, auto-dilution, and auto-reflex testing Automation-ready Point-in-space aspiration enables connectivity to a track. Redundant sample loading provides built-in backup and easy handling of non-routine samples. Direct sampling from track prevents tubes being detained at an analyzer and reduces aliquoting. Minimal Need for Operator Intervention Minimal-maintenance ISEs Large onboard reagent capacity and optional concentrated reagents for fewer interruptions No-maintenance, 37°C incubation oil bath Auto-calibration and auto-QC from refrigerated onboard calibrators and controls Microvolume technology with 32,000 tests onboard and up to 60 days' onboard stability for improved walkaway time. Concentrated reagents for increased maximum capacity. Remote diagnostics and Inter-Laboratory Quality Control (ILQC) options Exceptional Reliability Sample integrity check for icterus, hemolysis, and lipemia Sample probe with automatic clot/clog detection, liquid-level sensing, short-sample detection, and crash protection Advances in onboard reagent stability, calibration frequency, reduction in interferences, and expanded assay linearity

ADVIA Chemistry XPT System An advanced, automated clinical chemistry analyzer for high-volume laboratories The ADVIA® Chemistry XPT System provides timely, reliable results with continuous operation to meet the chemistry workloads of the most demanding laboratories. Delivers high throughput of up to 2400 tests per hour. Manages comprehensive testing with a menu of more than 115 assays. Improves workflow using automation and system technologies such as micro-volume and aliquot retention. Features & Benefits The ADVIA Chemistry XPT System*** is engineered for continuous operation and timely, accurate results—supporting you to reach your full potential today and be prepared for tomorrow. With an advanced user interface that is easy to use and VeriSmart Technology to support accuracy of testing, the ADVIA Chemistry XPT System predictably and consistently delivers timely, reliable results to meet expanding workloads. Simplified, Continuous Operation Simplify operation and training with the intuitive, icon-driven user interface. Extend walkaway times with concentrated reagents that provide high test capacity onboard. Quickly load most system fluids on the fly. Automate calibration, QC, startup, and shutdown. Consistent, Predictable Turnaround Time and Accuracy Offer a comprehensive chemistry menu of over 115 assays, including general and specialty chemistry, DAT, TDM, and specific proteins. Push productivity with a throughput of up to 2400 tests per hour (1800 photometric, plus 600 ISE). Control testing: STAT and specialty samples can always be front-loaded while an automation track is running. Designed for Automation Adapt and grow with direct connectivity to Siemens Healthineers and . Simplify your laboratory operations with connectivity to IT solutions including , , and . Increase productivity and efficiency with direct-from-track sampling and onboard aliquoting that allows samples to be immediately released to the next workstation. Do More with Less Reduce blood-draw requirements with micro-volume technology that uses a single 30 μL sample for up to 15 tests. Eliminate sample callback with automated, pre-programmable dilution, reflex, and rerun testing that uses an onboard aliquot. Improve workflow and accuracy with VeriSmart echnologies that perform hardware and software checks at all stages of sample processing.

The Autochem Xact Pro is a Fully Automatic Random Access Clinical Chemistry Analyzer. It is a compact, benchtop analyser with throughput up to 200 tests per hour. First-in-Class On-board Warm-water Laundry System Probe with Collision-Protection System Multi function Probe which works as mixer also Probe with Capacitance based liquid level sensor Robust Multi-functional Software Intelligent" Mixing Arm Removable & flexible Sample - Reagent Disc with 40 Open position for reagent & samples respectively. DESIGNED FOR UNINTERRUPTED WORK FLOW OTHER FEATURES ROBUST MULTI-FUNCTIONAL SOFTWARE Probe with Collision-Protection System Both horizontal and vertical collision protection ensures that system re-starts testing after any unwanted physical intervention. This prevents wastage of reagents, saves test time and increases life of probe Multi functional Probe Probe has flexibility to work even as a mixer in case in-built mixer does not work Probe with Capacitance based liquid level sensor Probe with liquid level sensor checks and indicates the presence of adequate sample or reagent in the container before starting testing process. Thus prevents unwanted interruptions in testing process “Intelligent” Mixing Arm User defined, variable three level mixing speed ensure thorough mixing Accurate performance of regular and immunoturbidimetric assays Independent cleaning station for mixer which prevents carry-over ensuring high precision Removable & flexible Sample – Reagent Disc 40 Open position for reagent & samples respectively Facility to use primary & variable sample cup as well as non fixed position for Sample, Control, Calibrator & STAT samples Two bottle sizes of 18 mL or 5 mL with unique design to reduce reagent dead volume Optional internal Reagent & Sample barcode reader makes the operation user friendly & ensure correct result reporting Reaction tray 60 reusable plastic reaction cuvettes which is also scanned before performing the test which means less recurring cost without compromising on quality. To assure precise results Individual replacement of cuvette offer lower consumable cost REFLEX Testing Functionality Allows user to program Low & High panic value for a particular test which triggers auto-testing of assigned tests which ensures faster and comprehensive reporting Dynamic & Real-time display of Test Running Status for quicker decision making Running status of reagent tray, Sample tray & reaction tray Real-time monitoring of Reaction curve On board inventory management Real-time monitoring of reagent residual volume saves reagent cost & time In-built full QC Program Provision to run 3 levels of control with Levey Jennings graph which gives confidence in reporting with multiple rules (1SD, 2SD and 3SD) Reagent, Calibrator & Control lot monitoring User friendly Bi-Directional LIS Interface

Best Fully Automated Clinical Chemistry Analyzer – Sphera M from Accurex. This chemistry analyzer is made in Italy & provides Accurate, Precise and Rapid Testing Solution. Sphera M is a Compact, fully automated, open random-access analyzer. The Minimum reaction volume is 210 µl, Minimal < 1L/h water consumption, 80 reusable UV transmitting Bionex® plastic cuvettes, 8-step wash station. It has Removable refrigerated tray with 30 open reagent positions for 50 ml or 20 ml bottles. Multiple virtual reagent disks can be programmed. The Chemistry analyser also does Auto-wash of inner & exterior probe surface. QUALITY CONTROL & CALIBRATION QUALITY CONTROL: • Levey-Jennings plots and Westgard multi rules • Upto 3 QC levels per test • QC monitor and reports • Method statistics: Test counter, CV%, mean, graphical trend analysis CALIBRATION: • Method and reagent monitor (volumes, available tests, calibration, QC status) • Automatic pre-dilution for calibrators • Upto 8 calibrators per method • Auto recalculation of result using last calibration. UNIQUE SOFTWARE DESIGN AND ARCHITECTURE: • Software designed for touch screen use • Intuitive user interface • Continuous loading of samples and reagents • Random-access and STAT • Free choice of execution order • Extended walk-away capability • Results archive with cumulative charts • Programmable automatic start up routine • Multiple work lists INTERFACE: • Bi-directional LIS, ASTM, over ethernet port of the external PC • Positive identification of samples with internal barcode reader (optional) • Printing on any Windows 7®/8® compatible printer • Method defined washing programs SYSTEM PACKS FOR SPHERA™ Accurex has developed dedicated system packs for Sphera™. Our biochemistry reagents have been intensively evaluated and validated to achieve the highest performance in Sphera™. Accurex recommends the use of these system packs according to instructions and applications validated by Accurex. Safety Information 8-step wash station for thorough cleaning of each cuvette (Needles: 6 dispensing, 6 aspiration, 1 cleaning) • Two types of systemic and special washing solutions for removing obstinate probe residues Direction for Use QUALITY CONTROL • Levey-Jennings plots and Westgard multi rules • Upto 3 QC levels per test • QC monitor and reports • Method statistics: Test counter, CV%, mean, graphical trend analysis CALIBRATION • Method and reagent monitor (volumes, available tests, calibration, QC status) • Automatic pre-dilution for calibrators • Upto 8 calibrators per method • Auto recalculation of result using last calibration Other Info Accurex has developed dedicated system packs for Sphera™. Our biochemistry reagents have been intensively evaluated and validated to achieve the highest performance in Sphera™. Accurex recommends the use of these system packs according to instructions and applications validated by Accurex.

Buy the Best Automated Hematology Analyzers – Acculab CBC 360 Neo. This Hematology Analyser Machine is Truly Automated for self-checking, background test, calibration, sampling, dilution, mixing, printing, dormancy, alarms, cleaning and maintenance. CBC 360 Neo is 3 part hematology analyzer from Accurex & is Automated for Walk-away Operations. Dual Chamber: Measurement of RBC / platelets & WBC / HGB in different chambers ensure accurate counting in a short time. The micro-aperture sizes of both the chambers are well designed to avoid error due to cell misclassification Automation for Walk-away Operation: Auto Self-check : Automatic aspiration of reagents & rinsing of tubings on startup Auto Blank : Background test is run automatically on startup Auto Print : Automatic printing of report with / without histogram Auto Sleep : Dormancy status is entered after a set period of non-operation Auto Alarms : Audio / visual alarms in case of system errors & abnormal results Auto Clean : Automatic rinsing options available to maintain the system Dual Chamber: Measurement of RBC / platelets & WBC / HGB in different chambers ensure accurate counting in a short time. The micro-aperture sizes of both the chambers are well designed to avoid error due to cell misclassification Specifications: Measurement principle: Electrical resistance for counting RBC, WBC & Platelets Colorimetric estimation of HGB at 540 nm Parameters: WBC, LY, MO, GR, LY%, MO%, GR%, RBC, HGB, HCT, MCV, MCH, MCHC, RDW-CV, RDW-SD, PLT, MPV, PDW, PCT, P-LCR Throughput: 60 samples/hour Micro-aperture: WBC : 100 um RBC / PLT : 68 um Sample type: Whole blood or pre-diluted sample Sample volume: Whole blood (venous blood) : 10 ul Chamber: Pre-diluent (capillary blood) : 20 ul, Pre-diluent (capillary blood) : 20 ul Reagents: Dual chamber : RBC / Platelets & WBC / HGB Quality control: 4 QC options : L-J, X-B, X-R & X QC, Graphs plotted with 31 data points Data memory: Upto 100,000 (with histogram) Display: 10.4 inch LCD with 640 x 480 resolution Printer: Built-in thermal printer Interface: Support RS-232C, standard network port and USB; keyboard and mouse Operating environment: Temperature : 15˚C – 35˚C, Humidity : 85% RH Electrical specification: Voltage : AC 110V/230V 23V Frequency : 50/60 Hz, Power : 180W Fuse specification : 250V/3A Dimensions / Weight: 670 x 515 x 640 mm / 27.5 kgs Specification Acculab CBC 360 Neo – Fully Automated Hematology Analyzer Features: 3-part differentiation of WBC Automated for — self-checking, background test, calibration, sampling, dilution, mixing, printing, dormancy, alarms, cleaning and maintenance High processing speed : 60 samples/hour Multi-parameter estimation : 20 parameters categorized according to corpuscle type for easy reporting Direct command keys for Mode, Prime, Flush, Drain & Record / Print for faster operation Run / Standby indicator lights denote the analyzer status i.e. test is being run or analyser is ready to test Whole blood/ Pre-diluent indicator lights denote the sample type in use i.e. whole blood/ pre-diluent Dual sample mode with low sample volume: Whole blood (venous) mode : 10 ul; pre-diluent (capillary) mode : 20 ul Audio alarm indication for abnormal findings or system errors 4 QC methods : L-J, X-B, X-R & X QC Comprehensive QC graphs with 31 data points for each parameter Internal & external probe cleaning facility Dormancy mode available to reduce power consumption Memory : 100,000 (with histogram) Safety Information • Large 10.4 inch single screen color display of all 21 parameters and 3 histograms • Sequential arrangement of WBC, RBC & PLT parameters for convenient reporing •Easy to understand & icon-based arrangement of menu for fast and convenient testing Direction for Use • WBC Histogram: R1, R2, R3, R4 & RM indications on histogram show specific abnormalities in the histogram & the probable causes • Platelet Histogram: PM indication is given when the boundary between PLT & RBC is ill-defined thus avoiding misclassification Other Info High voltage cautery function is useful in disintegrating obstinate protein or serum clog in the tubing